Lipoprotein Metabolism Regulation by Sterols

We obtained lovastatin (PHR1285), mevalonate (41288), 25-hydroxycholesterol (H1015), MG-132 (M8699), Filipin (F9765), geranylgeraniol (G3278), protease inhibitor cocktail (P8340), N-Ethylmaleimide (E3876), and paraformaldehyde (PFA) (P6148) from Sigma; [¹⁴C]-acetic acid sodium salt (NEC084H001) from Perkin Elmer; and FuGENE HD transfection reagent (E2312) from Promega; G418 (345810), digitonin (300410), henylmethylsulfonyl fluoride (PMSF) (52332), leupeptin (108975), pepstatin A (516481) and ALLN (208719) from Merck; Hoechst 33342 (H1399) from Invitrogen. Lipoprotein-deficient serum (LPDS) [41 (link)] and delipidated-fetal calf serum (FCS) [42 (link)] was prepared from FCS (S1580, Biowest) by ultracentrifugation in our laboratory [25 (link)].

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Jiang S.Y., Tang J.J., Xiao X., Qi W., Wu S., Jiang C., Hong J., Xu J., Song B.L, & Luo J. (2019). Schnyder corneal dystrophy-associated UBIAD1 mutations cause corneal cholesterol accumulation by stabilizing HMG-CoA reductase. PLoS Genetics, 15(7), e1008289.

Publication 2019

lovastatin paraformaldehyde (PFA) (P6148) FuGENE HD transfection reagent ( Hoechst 33342 (H1399)

25 hydroxycholesterol Acetic acid Digitonin Fetal calf serum Filipin Fluoride Fugene G418 Geranylgeraniol Hoechst 33342 Leupeptin Lipoprotein Lovastatin Mevalonate Mg 132 N ethylmaleimide Paraformaldehyde Pepstatin Promega Protease inhibitor Salt Serum Sodium Transfection Ultracentrifugation

Corresponding Organization : City University of Hong Kong, Shenzhen Research Institute

Other organizations : Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, ShanghaiTech University

Top 5 similar protocols

Variable analysis

independent variables

Lovastatin (PHR1285)
Mevalonate (41288)
25-hydroxycholesterol (H1015)
MG-132 (M8699)
Filipin (F9765)
Geranylgeraniol (G3278)
N-Ethylmaleimide (E3876)

dependent variables

Not explicitly mentioned

control variables

Protease inhibitor cocktail (P8340)
Paraformaldehyde (PFA) (P6148)
[¹⁴C]-acetic acid sodium salt (NEC084H001)
FuGENE HD transfection reagent (E2312)
G418 (345810)
Digitonin (300410)
Henylmethylsulfonyl fluoride (PMSF) (52332)
Leupeptin (108975)
Pepstatin A (516481)
ALLN (208719)
Hoechst 33342 (H1399)
Lipoprotein-deficient serum (LPDS)
Delipidated-fetal calf serum (FCS)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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