Tissue metabolite extraction and derivatization were performed as described in Gaglio et al. [50 (link)]. GC/MS analysis was performed using 7200 accurate-mass Q-TOF GC/MS (Agilent Technologies) equipped with a 40-m DB-35MS capillary column operating under electron impact (EI) ionization at 70eV. Samples (1 μl) were injected in a splitless mode at 250°C, using helium as the carrier gas at a flow rate of 1 ml/min. The GC oven temperature was held at 100°C for 2 min and increased to 325°C at 10°C/min. GC/MS data processing was performed using Agilent Muss Hunter software and statistical analyses were performed using Mass Profiler Professional (MPP) software.
Valtorta S., Lo Dico A., Raccagni I., Gaglio D., Belloli S., Politi L.S., Martelli C., Diceglie C., Bonanomi M., Ercoli G., Vaira V., Ottobrini L, & Moresco R.M. (2017). Metformin and temozolomide, a synergic option to overcome resistance in glioblastoma multiforme models. Oncotarget, 8(68), 113090-113104.
Other organizations :
Institute of Molecular Bioimaging and Physiology, University of Milan, Vita-Salute San Raffaele University, Istituti di Ricovero e Cura a Carattere Scientifico, University of Massachusetts Chan Medical School, Boston Children's Hospital, University of Milano-Bicocca, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
Tissue metabolite extraction and derivatization procedure
dependent variables
Metabolite levels measured by GC/MS
control variables
GC/MS equipment and parameters (7200 accurate-mass Q-TOF GC/MS, 40-m DB-35MS capillary column, electron impact ionization at 70eV, splitless injection at 250°C, helium carrier gas at 1 ml/min, GC oven temperature program)
Data processing software (Agilent Muss Hunter)
Annotations
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