Viral RNA Extraction and Quantification

Viral RNA was extracted from serum and CSF using the High Pure Viral RNA Kit (Roche). Semen, as well as the indicated lymphoid, reproductive, gastrointestinal (GI), and neural tissues were homogenized in Qiazol (Qiagen) using either disposable tissue grinders (Fisherbrand) or a TissueRuptor (Qiagen), and RNA was isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen). Viral RNA from body fluids and tissues was quantified using qRT-PCR as described previously^{12 (link)}.

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Schouest B., Fahlberg M., Scheef E.A., Ward M.J., Headrick K., Szeltner D.M., Blair R.V., Gilbert M.H., Doyle-Meyers L.A., Danner V.W., Bonaldo M.C., Wesson D.M., Panganiban A.T, & Maness N.J. (2020). Immune outcomes of Zika virus infection in nonhuman primates. Scientific Reports, 10, 13069.

Publication 2020

High Pure Viral RNA Kit Qiazol disposable tissue grinders TissueRuptor RNeasy Lipid Tissue Mini Kit

Body fluids Lipid Lymphoid Neural tissues Reproductive Semen Serum Tissues Viral rna

Corresponding Organization :

Other organizations : Tulane University, Fundação Oswaldo Cruz

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Variable analysis

independent variables

Extraction method (High Pure Viral RNA Kit, Qiazol homogenization, RNeasy Lipid Tissue Mini Kit)

dependent variables

Viral RNA quantity in serum, CSF, semen, lymphoid, reproductive, gastrointestinal (GI), and neural tissues

control variables

Not explicitly mentioned

controls

No positive or negative controls specified

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