All reagents were purchased from Sigma Aldrich unless otherwise indicated. ERK inhibitor SCH772984 (14 (link)) was purchased from Active Biochem and used at a concentration of 1000 nM for in vitro experiments. The MEK inhibitor trametinib was purchased from Selleckchem and used at a concentration of 100 nM for in vitro experiments. All drugs were reconstituted in sterile, dry DMSO, aliquoted, and stored at 80 °C until further use.
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Inhibitors of ERK and MEK Signaling
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Corresponding Organization : Cornell University
Other organizations : Rockefeller University
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Variable analysis
independent variables
- Concentration of SCH772984 (1000 nM)
- Concentration of trametinib (100 nM)
- Not explicitly mentioned
- All reagents were purchased from Sigma Aldrich unless otherwise indicated
- Drugs were reconstituted in sterile, dry DMSO, aliquoted, and stored at 80 °C until further use
- No positive or negative controls were explicitly mentioned
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