Most compounds used in the estrogen receptor library screen were synthesized in-house and have been reported.32 (link)–35 (link), 56 (link), 57 (link) Toremifene, tamoxifen, raloxifene, bazedoxifene, ospemifene, ridaifen-B, nafoxidine, RAD-1901, MPP, acolbifene, and compound 7 were purchased from Sigma-Aldrich or Med Chem Express. All compounds were dissolved in DMSO at a final concentration of 10 mM and were stored at −20 °C until use.
Protocol detail
Estrogen Receptor Library Screening
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Acolbifene
Bazedoxifene
Dmso
Estrogen receptor
Library
Nafoxidine
Ospemifene
Raloxifene
Ridaifen b
Tamoxifen
Toremifene
Corresponding Organization : University of Illinois at Chicago
Other organizations : Boston University, Texas Biomedical Research Institute
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Variable analysis
independent variables
- Compounds used in the estrogen receptor library screen
- Not explicitly mentioned
- DMSO concentration (10 mM)
- Storage temperature (-20 °C)
- Positive controls: Toremifene, tamoxifen, raloxifene, bazedoxifene, ospemifene, ridaifen-B, nafoxidine, RAD-1901, MPP, acolbifene, and compound 7
- Negative control: Not specified
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