Serial sections of three-micrometer-thick formalin-fixed, paraffin-embedded tissue sections were handled as described (7 (link)). Incubation conditions for the primary antibodies are included in Supplementary Table 1 . EnVision™ system-horseradish peroxidase (HRP)-labeled polymer anti-mouse (Dako, ref. K4000) for mouse primary antibodies and EnVision™ system-HRP-labeled polymer anti-rabbit (Dako, ref. K4011) for rabbit primary antibodies were incubated for 40 min at room temperature.
For the negative controls, the same protocols were followed, but the primary antibody was omitted to avoid non-specific signals.
For the negative controls, the same protocols were followed, but the primary antibody was omitted to avoid non-specific signals.
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