Mice were sacrificed by intraperitoneal (i.p.) administration of 90 mg/kg body weight sodium pentobarbital. Bronchoalveolar lavage fluid (BALF) was obtained by lavaging the lungs with 3 mM EDTA in PBS [32 (link)] and the cellular composition was determined by hemocytometer cell counts and differential counts of cytospins after staining with Quick-Diff solution (Siemens; Medical Solutions Diagnostics, Tarrytown, NY, USA).
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