Quantifying DNA Damage via Flow Cytometry

p-Histone H2AX antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used to detect γH2AX. Subsequently, fixed cells were incubated with Alexa Fluor^® 488-secondary antibody (Cell Signaling Technology, Danvers, MA, USA) [33 (link),34 (link)], accompanied by 7AAD (1 μg/mL, 30 min) incubation. 8-OHdG-FITC antibody (1:10,000 dilution) (Santa Cruz Biotechnology) was used to detect 8-OHdG [35 ]. Finally, cells were resuspended in 1× PBS for flow cytometry.

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Liu W., Hsu Y.Y., Tang J.Y., Cheng Y.B., Chuang Y.T., Jeng J.H., Yen C.H, & Chang H.W. (2022). Methanol Extract of Commelina Plant Inhibits Oral Cancer Cell Proliferation. Antioxidants, 11(9), 1813.

Publication 2022

p-Histone H2AX antibody Alexa Fluor® 488-secondary antibody 8-OHdG-FITC antibody

7aad 8 ohdg Alexa fluor 488 Antibody Cells Dilution Fitc Flow cytometry Histone h2ax

Corresponding Organization : National Sun Yat-sen University

Other organizations : National Taiwan University Hospital

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Variable analysis

independent variables

P-Histone H2AX antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA)
Alexa Fluor® 488-secondary antibody (Cell Signaling Technology, Danvers, MA, USA)
7AAD (1 μg/mL, 30 min)
8-OHdG-FITC antibody (1:10,000 dilution) (Santa Cruz Biotechnology)

dependent variables

γH2AX
8-OHdG

control variables

Fixed cells

positive controls

No positive controls mentioned

negative controls

No negative controls mentioned

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