TOP/FOP-Flash assay was carried out as described previously24 (link). In short, cells were cotransfected with TOP/FOP-Flash (Genechem) and different plasmids for SOX9 overexpression, SOX9 inhibition, or FARSA-AS1 inhibition. After normalizing to the Renilla luciferase activity, the TOP/FOP ratio was measured via dual luciferase reporter system (Promega).
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