Lineage Scorecard for Directed EB Differentiation

iPSCs were cultured and dissociated as described for nEBs above. nEBs were formed in hES media without FGF for 16 days with media changes every two days. nEBs were harvested and RNA was collected using a MicroRNAeasy kit (Qiagen, 217004). Lineage scorecard was calculated for nondirected EB differentiation using the NanoString probe set described by Bock et al. after quantification using single-molecule imaging on an nCounter System according to the manufacturer’s instructions by the Meissner Lab at Harvard University.^{37 (link)}

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Madison J.M., Zhou F., Nigam A., Hussain A., Barker D.D., Nehme R., van der Ven K., Hsu J., Wolf P., Fleishman M., O’Dushlaine C., Rose S., Chambert K., Lau F.H., Ahfeldt T., Rueckert E.H., Sheridan S.D., Fass D.M., Nemesh J., Mullen T.E., Daheron L., McCarroll S., Sklar P., Perlis R.H, & Haggarty S.J. (2015). Characterization of Bipolar Disorder Patient-Specific Induced Pluripotent Stem Cells from a Family Reveals Neurodevelopmental and mRNA Expression Abnormalities. Molecular psychiatry, 20(6), 703-717.

Publication 2014

MicroRNAeasy kit

Ipscs Nebs

Corresponding Organization :

Other organizations : Center for Human Genetics, Broad Institute, Massachusetts General Hospital, Harvard University, Icahn School of Medicine at Mount Sinai

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Variable analysis

independent variables

Culture and dissociation of iPSCs
Formation of nEBs in hES media without FGF for 16 days

dependent variables

Gene expression profiles of differentiated nEBs

control variables

Media change every two days during nEB formation

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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