Transient Agrobacterium‐mediated transformations of corollas of wild type (WT) and pODO1:GFP‐ODO1 line 10 (T2) with 35S:NLS‐mCherry were performed as described previously (Moerkercke et al., 2012 (link); Verweij et al., 2008 (link)). After 2 days, GFP (505–550 nm bandpass emission filter, 488 nm excitation) and mCherry (585–615 nm bandpass emission filter, 561 nm excitation) were imaged with a Zeiss LSM 510 inverted microscope (Zeiss, https://www.zeiss.com ). Images were analyzed with imagej (https://fiji.sc ).
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Transient Agrobacterium-mediated Transformation
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Corresponding Organization : University of Amsterdam
Other organizations : Inholland University of Applied Sciences, Purdue University West Lafayette
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Variable analysis
independent variables
- Transient Agrobacterium-mediated transformations
- Wild type (WT) and pODO1:GFP-ODO1 line 10 (T2) corollas
- 35S:NLS-mCherry
- GFP expression (505–550 nm bandpass emission filter, 488 nm excitation)
- MCherry expression (585–615 nm bandpass emission filter, 561 nm excitation)
- Transformation protocol (described previously in Moerkercke et al., 2012 and Verweij et al., 2008)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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