Immunohistochemical Analysis of Murine Pancreatic Tumors

IHC was conducted with reference to previous studies (Liao et al., 2019 (link); Wu et al., 2020 (link)). Paraffin sections of mouse pancreatic tumors were subjected to a standard xylene and alcohol gradient for deparaffination, and then antigen retrieval was carried out through Citrate Antigen Retrieval solution (Solarbio, China). Streptavidin-Biotin Assay Kit (ZSGB-BIO, China) was used for endogenous peroxidase blockade and antibody incubation. Sections were incubated with primary antibodies, including anti-Ki67 antibody (Beyotime, China) and anti-Ly-6G/Ly-6C (Gr-1) antibody (BioLegend, United States). After overnight at 4°C, sections were incubated with Biotin-conjugated Goat Anti-Rat or Rabbit IgG (Proteintech, United States). Sections were subsequently coloured by using DAB kit (ZSGB-BIO, China) and stained with hematoxylin. The expression of Gr-1 and Ki67 was quantitatively analyzed using ImageJ Fiji software according to the previous protocol (Crowe and Yue, 2019 (link)).

Free full text: Click here

Wang B., Yang L., Liu T., Xun J., Zhuo Y., Zhang L., Zhang Q, & Wang X. (2021). Hydroxytyrosol Inhibits MDSCs and Promotes M1 Macrophages in Mice With Orthotopic Pancreatic Tumor. Frontiers in Pharmacology, 12, 759172.

Publication 2021

Citrate Antigen Retrieval solution anti-Ki67 antibody DAB kit

Alcohol Anti antibody Antibodies Antibody Antigen Assay Biotin Citrate Goat Mouse Pancreatic tumors Paraffin Peroxidase Rabbit Streptavidin Xylene

Corresponding Organization : Tianjin University

Other organizations : Tianjin Nankai Hospital

Top 5 similar protocols

Variable analysis

independent variables

Paraffin sections of mouse pancreatic tumors

dependent variables

Expression of Gr-1 and Ki67

control variables

Standard xylene and alcohol gradient for deparaffination
Citrate Antigen Retrieval solution for antigen retrieval
Streptavidin-Biotin Assay Kit for endogenous peroxidase blockade and antibody incubation
Primary antibodies: anti-Ki67 antibody and anti-Ly-6G/Ly-6C (Gr-1) antibody
Biotin-conjugated Goat Anti-Rat or Rabbit IgG
DAB kit for coloring and hematoxylin for staining

controls

No positive or negative controls were explicitly mentioned in the protocol.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!