Gene expression profiling of BxPC3 and BxPC3-R cells was performed using GeneChip miRNA 3.0 Array (Affymetrix, Santa Clara, CA). Cellular RNA enriched for small RNAs (<200 nt) was isolated using the mirVana miRNA isolation kit according to the manufacturer's instructions (ThermoFisher Scientific). The concentration and quality of miRNA were determined using the Small RNA Assay in the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). In all, 400 ng of cellular RNA enriched for small RNAs was labeled using the FlashTag Biotin HSR Labeling Kit, hybridized for 18 h at 48 °C (rotation 60 r.p.m.) on the GeneChip miRNA 3.0 Array, washed in the Affymetrix Fluidics Station 450 and scanned using the Affymetrix GeneChip Scanner 3000 7G (all from Affymetrix) as previously described.12 (link) Microarray experiments were performed in two replicates per each cell type. The data were analyzed with Expression Console software Build 1.2.1.20 (Affymetrix) using the default analysis setting for RMA+DAGB workflow and submitted to the Gene Expression Omnibus repository (GEO, available under the series accession number GSE79506).