Molecular Identification of Influenza Subtypes

Viral RNA was extracted using a Miracle-AutoXT Automated Nucleic Acid Extraction System (iNtRON Biotechnology, Seongnam, Korea). To identify subtypes, qRT-PCR was conducted using the TOPreal™ One-step RT qPCR Kit (Enzynomics, Daejeon, Korea) with influenza-specific primers as previously described [35 (link)]. Species identification was performed using mitochondrial cytochrome C oxidase I mitochondrial gene in the DNA of fecal samples, as previously described [36 (link)].

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Na E.J., Kim Y.S., Kim Y.J., Park J.S, & Oem J.K. (2021). Genetic Characterization and Pathogenicity of H7N7 and H7N9 Avian Influenza Viruses Isolated from South Korea. Viruses, 13(10), 2057.

Publication 2021

Miracle-AutoXT Automated Nucleic Acid Extraction System TOPreal™ One-step RT qPCR Kit

Cytochrome c oxidase Fecal Gene Influenza Intron Mitochondrial dna Mitochondrial gene Nucleic acid Primers Viral rna

Corresponding Organization : Jeonbuk National University

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Variable analysis

independent variables

RNA extraction method (Miracle-AutoXT Automated Nucleic Acid Extraction System)
QRT-PCR assay (TOPreal™ One-step RT qPCR Kit)

dependent variables

Influenza subtype identification
Species identification using mitochondrial cytochrome C oxidase I gene

control variables

Influenza-specific primers as previously described [35]
Mitochondrial cytochrome C oxidase I gene as previously described [36]

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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