To measure mitochondrial superoxide and the mitochondrial membrane potential state, cells were labelled with 5 μM MitoSOX Red mitochondrial superoxide indicator reagent (ThermoFisher) or with 100 nM TMRM (ThermoFisher) and were incubated for 30 min at 37 ºC. Cells then were washed with PBS prior to cell surface staining as described above and analyzed by for flow cytometry.
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Mitochondrial Superoxide and Membrane Potential
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Other organizations : Institut National de la Recherche Scientifique, McGill University
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Variable analysis
independent variables
- Incubation with 5 μM MitoSOX Red mitochondrial superoxide indicator reagent
- Incubation with 100 nM TMRM
- Mitochondrial superoxide
- Mitochondrial membrane potential state
- Incubation time of 30 min at 37 ºC
- Washing with PBS prior to cell surface staining
- Analysis by flow cytometry
- No positive or negative controls were explicitly mentioned in the input protocol.
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