Adipogenic Differentiation of Cell Lines

Three cell lines that represent different stages of adipogenesis were used: adipose-derived stem cells (ADSCs), human preadipocytes (HPAd), and human adipocytes (HAd). StemPro human ADSCs (Life Technologies) have the same phenotypic and functional characteristics as bone marrow-derived mesenchymal stem cells. ADSCs were maintained in MesenPRO RS basal medium plus Growth Supplement. HPAd were cultured in preadipocyte growth medium, whereas HAd were maintained in adipocyte differentiation medium according to the manufacturer’s instructions (Cell Application, Inc.).
Adipogenic differentiation was induced in Poietics bone marrow-derived hMSCs (Lonza) according to the manufacturer’s instructions. The PLS-derived cell line LS226 (link) was maintained in RPMI plus GlutaMAX (Life Technologies), 1× MEM Essential Vitamin Mix (BioWhittaker), 1× ITES (BioWhittaker), 1× penicillin/streptomycin/L-glutamine (Life Technologies), 1 mM sodium pyruvate (CellGro), 1× nonessential amino acids (NEAA) (Lonza), and 20% fetal bovine serum (Life Technologies).

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Shaker M., Pascarelli K.M., Plantinga M.J., Love M.A., Lazar A.J., Ingram D.R., von Mehren M., Lev D., Kipling D, & Broccoli D. (2014). Differential Expression of Cysteine Dioxygenase 1 in Complex Karyotype Liposarcomas. Biomarkers in Cancer, 6, 1-10.

Publication 2014

penicillin/streptomycin/L-glutamine sodium pyruvate nonessential amino acids (NEAA) fetal bovine serum

Adipocyte Adipogenic Amino acids Bone marrow Bone marrow mesenchymal stem cells Cell Cell line Fetal bovine serum Glutamine Human Penicillin Phenotypic Pyruvate Sodium Stem Streptomycin Supplement Vitamin

Corresponding Organization :

Other organizations : Memorial Health University Medical Center, Mercer University, The University of Texas MD Anderson Cancer Center, Fox Chase Cancer Center, Cardiff University

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Variable analysis

independent variables

Adipogenic differentiation induction in Poietics bone marrow-derived hMSCs (Lonza) according to the manufacturer's instructions
Cell lines representing different stages of adipogenesis: adipose-derived stem cells (ADSCs), human preadipocytes (HPAd), and human adipocytes (HAd)

dependent variables

Not explicitly mentioned

control variables

Culture conditions for each cell line:
- ADSCs maintained in MesenPRO RS basal medium plus Growth Supplement
- HPAd cultured in preadipocyte growth medium
- HAd maintained in adipocyte differentiation medium
LS226 cell line maintained in RPMI plus GlutaMAX, 1× MEM Essential Vitamin Mix, 1× ITES, 1× penicillin/streptomycin/L-glutamine, 1 mM sodium pyruvate, 1× nonessential amino acids, and 20% fetal bovine serum

controls

Positive control: Adipogenic differentiation of Poietics bone marrow-derived hMSCs (Lonza) according to the manufacturer's instructions
Negative control: Not explicitly mentioned

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