A micro-sample genomic DNA extraction kit (DP316, Tiangen) was used to extract the nucleic acid. NEBNext Ultra II DNA Library Prep Kit (New England Biolabs Inc.) was used to construct Illumina sequencing libraries and Nextseq 550 DX (75 bp single-end reads; Illumina) was used for sequencing. An alignment tool (Burrows-Wheeler Alignment) was used to map to a human reference genome (GRCh38) to exclude human sequence data. The remaining sequencing data were aligned to NCBI nt database by SNAP. The specific detection method of mNGS can be referred to our previous report (Zhang et al., 2022 (link)).
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