Immunization of Mice for Calcium Flux

Mice were immunized in bilateral foot pads with 100 μl of a mixture of 100 μg papain (Sigma-Aldrich) and 100 μg 4-hydroxy-3-nitrophenylacetyl conjugated to ovalbumin (NP-ova; Sigma-Aldrich). After 5 d, inguinal and popliteal lymph nodes were harvested. Lymphocytes were prepared for calcium flux analysis as described below.

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Seth A., Yokokura Y., Choi J.Y., Shyer J.A., Vidyarthi A, & Craft J. (2023). AP-1–independent NFAT signaling maintains follicular T cell function in infection and autoimmunity. The Journal of Experimental Medicine, 220(5), e20211110.

Publication 2023

papain NP-ova

Calcium Foot Inguinal Lymph nodes Lymphocytes Mice Ovalbumin Papain

Corresponding Organization : Yale University

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Variable analysis

independent variables

Immunization with a mixture of 100 μg papain (Sigma-Aldrich) and 100 μg 4-hydroxy-3-nitrophenylacetyl conjugated to ovalbumin (NP-ova; Sigma-Aldrich)

dependent variables

Calcium flux analysis of lymphocytes from inguinal and popliteal lymph nodes

control variables

Bilateral foot pads for immunization
Lymphocyte preparation for calcium flux analysis as described

controls

No positive or negative controls were explicitly mentioned in the provided information.

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