Assessing Exploratory Behavior in Mice

Both scores were assessed before any other assessment or handling of the mice. To examine the motivation of the mice to explore and interact (sniffing, holding with forepaws, or carrying) with a foreign object, we added a Nestlet (Ancare, Bellmore, USA) to the home cages and observed the mice for one minute. The explorative test was scored 1 (interaction) or 0 (no interaction) per cage. An interaction of one animal of the cage was deemed as sufficient for a positive score. Nest complexity scoring was performed following Hess et al.^{68 (link)} in the home cage assigning scores between 0 and 5.

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Wolter A., Bucher C.H., Kurmies S., Schreiner V., Konietschke F., Hohlbaum K., Klopfleisch R., Löhning M., Thöne-Reineke C., Buttgereit F., Huwyler J., Jirkof P., Rapp A.E, & Lang A. (2023). A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models. Scientific Reports, 13, 3824.

Publication 2023

Nestlet

Animal Foreign object Mice Motivation

Corresponding Organization : Freie Universität Berlin

Other organizations : Berlin Institute of Health at Charité - Universitätsmedizin Berlin, University of Basel, German Rheumatism Research Centre, University of Zurich

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Variable analysis

independent variables

Adding a Nestlet (Ancare, Bellmore, USA) to the home cages

dependent variables

Exploratory behavior (interaction or no interaction with the Nestlet)
Nest complexity (scored between 0 and 5)

control variables

The scores were assessed before any other assessment or handling of the mice

controls

No positive or negative controls were explicitly mentioned in the provided information.

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