Quantifying SARS-CoV-2 Viral Load in Non-Human Primates
Viral load was quantified in pharyngeal and nasal swabs as well as supernatant and cells from BAL for the animals housed at TNPRC using RT-qPCR. Probes targeting the nucleocapsid or envelope gene were used to quantify both genomic and subgenomic RNA of SARS-CoV-2. A Zymo Quick RNA Viral Kit (#R1035 or #D7003), Zymo, USA) was used to isolate RNA according to manufacturer’s protocol and as previously described (17 (link)). RNA was analyzed using a QuantStudio 6 (Thermo Scientific, USA) and TaqPath master mix (Thermo Scientific, USA) as previously described (17 (link)).
Madden P.J., Arif M.S., Becker M.E., McRaven M.D., Carias A.M., Lorenzo-Redondo R., Xiao S., Midkiff C.C., Blair R.V., Potter E.L., Martin-Sancho L., Dodson A., Martinelli E., Todd J.P., Villinger F.J., Chanda S.K., Aye P.P., Roy C.J., Roederer M., Lewis M.G., Veazey R.S, & Hope T.J. (2021). Development of an In Vivo Probe to Track SARS-CoV-2 Infection in Rhesus Macaques. Frontiers in Immunology, 12, 810047.
Corresponding Organization : Northwestern University
Other organizations :
Tulane University, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Discovery Institute, Sanford Burnham Prebys Medical Discovery Institute, University of Louisiana at Lafayette
Viral load quantified in pharyngeal and nasal swabs as well as supernatant and cells from BAL using RT-qPCR
control variables
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controls
Positive control: Not specified
Negative control: Not specified
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