Signaling Pathway Modulation in PEDV

p38 inhibitor SB203580 (10 μM), c-Jun N-terminal kinase (JNK) inhibitor SP600125 (10 μM), extracellular signal-regulated kinase (ERK) inhibitor PD98059 (5 μM), PKA inhibitor H-89 (5 μM), c-Jun inhibitor SR11302 (5 μM), CREB inhibitor EML-425 (5 μM), and p53 inhibitor PFT-α (10 μM) are all purchased from MedChemExpress (Shanghai, China). All inhibitors were configured with dimethyl sulfoxide (DMSO; Solarbio, Beijing, China). Antibodies against Bax, Bcl-2, caspase 3, caspase 8, caspase 9, FasL, Fas, p53, his, phospho-p53(ser15), phospho-p53(ser46), and phospho-p53(ser20) were purchased from Cell Signaling Technology (Danvers, MA, United States). Monoclonal antibodies against CCN1, c-Jun, phospho-c-Jun, c-Fos, phospho-c-Fos, CREB, and phospho-CREB were obtained from ABclonal (Wuhan, China). Antibodies against PEDV (CH/SXYL/2016) N protein were stored in our laboratory (Xu et al., 2019 (link)).