For PMP-labeled monosaccharide analysis, the capillary HPLC separations were performed using a solvent system composed of acetonitrile (eluent A) and 0.01 M CH3COONH4 in water pH 5.5 (eluent B). After injection of 0.5 µl samples, the elution profile was 17% A for 10 min, 19% A for 30 min, 21% A for 15 minutes, and 17% A for 25 minutes. The flow rate was 10 µl per minute, the absorbencies at 245 nm were monitored during each run and the negative ion spectra were collected by using an online Thermo LTQ-XL mass spectrometer.
The same elution condition for capillary HPLC separation of aniline-labeled products was used as described by Lu et al [51] (link). Briefly, an Agilent 1290 series capillary HPLC workstation was coupled with Thermo LTQ-XL mass spectrometer. Negative ion spectra were collected by scanning the m/z range 100–1000. Total ion current chromatograms were collected. The mass spectra were processed with Thermo Xcalibur software.
The same elution condition for capillary HPLC separation of aniline-labeled products was used as described by Lu et al [51] (link). Briefly, an Agilent 1290 series capillary HPLC workstation was coupled with Thermo LTQ-XL mass spectrometer. Negative ion spectra were collected by scanning the m/z range 100–1000. Total ion current chromatograms were collected. The mass spectra were processed with Thermo Xcalibur software.
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