Quantification of MPO-DNA Complexes

Serum was collected for MPO-DNA testing at the time of venous thrombus harvesting. MPO-DNA complexes were quantified as we described previously (9 (link), 10 ). The protocol uses reagents from the Cell Death Detection ELISA kit (Roche) as well as an anti-MPO antibody (Bio-Rad0400–0002) that reacts with both human and mouse MPO.

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Ali R.A., Estes S.K., Gandhi A.A., Yalavarthi S., Hoy C.K., Shi H., Zuo Y., Erkan D, & Knight J.S. (2022). Defibrotide inhibits antiphospholipid antibody-mediated NET formation and venous thrombosis. Arthritis & rheumatology (Hoboken, N.J.), 74(5), 902-907.

Publication 2021

Cell Death Detection ELISA kit anti-MPO antibody

Anti antibody Cell death Elisa Human Mouse Serum Thrombus Venous

Corresponding Organization : University of Michigan–Ann Arbor

Other organizations : Hospital for Special Surgery, Cornell University

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Variable analysis

independent variables

Time of venous thrombus harvesting

dependent variables

MPO-DNA complexes

control variables

Use of reagents from the Cell Death Detection ELISA kit (Roche)
Use of an anti-MPO antibody (Bio-Rad0400-0002) that reacts with both human and mouse MPO

controls

Positive control: Not specified
Negative control: Not specified

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