Diabetes was induced by a single intravenous injection of 50 mg/kg body wt alloxan (Sigma) in saline (100 μL). The control group received an intravenous injection of saline (100 μL). Diabetic mice with a blood glucose level ≥300 mg/dL 3 days after alloxan treatment were used. In the fifth day, alloxan-treated animals were treated subcutaneously daily with saline or insulin for 5 days. One dose of bovine insulin (Sigma) was administered in the morning, and one dose of insulin glargine (long-acting insulin; sanofi-aventis) was administered at 5 p.m. Both were administered at a dose of 1 UI for each 200 mg/dL blood glucose. Blood samples were collected from the tail vein to measure blood glucose levels by a glucometer (Precision Xtra; Abbott). Ten days after alloxan treatment, mice were submitted to sham operation (SH), mild sepsis (MS), or severe sepsis (SS) by the cecum ligation and puncture (CLP) model and several parameters were analyzed. In another set of experiments, at day 10 after alloxan treatment, the mice were used for neutrophil migration assay in vivo or for blood neutrophil chemotaxis assay in vitro.