Molecular Markers of Metabolic Health

The primary antibodies against p-Akt (Ser473), Akt, p-Smad2, p-Smad3, PPARγ, p-ACC1 and PGC1-α were from Cell Signaling Technology (Beverly, MA). Antibodies against F4/80, CD68, Fndc5 and UCP-1 were from Abcam (Cambridge, MA). ELISA kits for measuring irisin, IL-6 or adiponectin were from BioVision (Milpitas, CA), eBioscience (San Diego, CA) or R&D Systems (Minneapolis, MN) respectively. The recombinant proteins, irisin or myostatin were obtained from Enzo life Sciences (Farmingdale, NY) or R&D systems. The anti-myostatin peptibody (peptibody) was from Atara Biotherapeutics (Westlake Village, CA) (21 (link);22 (link)). Serum insulin concentration was measured using the Rat/Mouse Insulin ELISA kit (Millipore, Billerica, MA). Serum free fatty acid levels were measured using the NEFA C kit from Wako (Richmond, VA).

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Dong J., Dong Y., Dong Y., Chen F., Mitch W.E, & Zhang L. (2015). Inhibition of myostatin in mice improves insulin sensitivity via irisin-mediated cross talk between muscle and adipose tissues. International journal of obesity (2005), 40(3), 434-442.

Publication 2015

p-Smad2 p-Smad3 PPARγ p-ACC1 PGC1-α Antibodies against F4/80, Fndc5 ELISA kits for measuring Irisin IL-6 adiponectin irisin myostatin Rat/Mouse Insulin ELISA kit NEFA C kit

Acc1 Adiponectin Antibodies Elisa Insulin Mouse Myostatin Nefa Pgc1 α Pparγ Recombinant proteins Serum Smad2 Smad3 Ucp 1

Corresponding Organization :

Other organizations : Sichuan University, Baylor College of Medicine

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Variable analysis

independent variables

Irisin
Myostatin
Anti-myostatin peptibody (peptibody)

dependent variables

P-Akt (Ser473)
P-Smad2
P-Smad3
PPARγ
P-ACC1
PGC1-α
F4/80
Fndc5
UCP-1
Irisin
Adiponectin
Serum insulin concentration
Serum free fatty acid levels

control variables

Not explicitly mentioned

positive controls

Not mentioned

negative controls

Not mentioned

Annotations

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