EPRS and STAT Signaling Regulation

All cytokines and growth factors including TGFβ1 were purchased from Peprotech (Rocky Hill, NJ, United States). Hydroxyproline assay kits, and CCl₄ were purchased from Sigma-Aldrich (St. Louis, MO, United States). Bleomycin and target specific pooled siRNAs siSTAT3 and siSTAT6 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, United States). EPRS in pEXPR-103-Strep vector (IBA Lifesciences, Göettingen, Germany) were gifts from Dr. Myung Hee Kim at the Korea Research Institute of Bioscience and Biotechnology (KRIBB, Daejeon, Korea). EPRS (1–1440 amino acids) consists of ERS (1–687 amino acids) and PRS (935–1,440 amino acids) linked via non-catalytic WHEP repeat domains (688–934 amino acids) (Ray and Fox, 2014 (link)). The PRS domain of EPRS was cloned into pEXPR-103-Strep vector (IBA Lifesciences). pRc/CMV-WT STAT3 was previously described (Choi et al., 2009 (link)) and pCMV-STAT6-IRES-Neo was a gift from Axel Nohturfft (Addgene plasmid # 35482). Adenovirus expressing SMAD2 or SMAD3 were described previously (Lee et al., 2005 (link)).

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Song D.G., Kim D., Jung J.W., Nam S.H., Kim J.E., Kim H.J., Kim J.H., Pan C.H., Kim S, & Lee J.W. (2018). Glutamyl-Prolyl-tRNA Synthetase Regulates Epithelial Expression of Mesenchymal Markers and Extracellular Matrix Proteins: Implications for Idiopathic Pulmonary Fibrosis. Frontiers in Pharmacology, 9, 1337.

Publication 2018

TGFβ1 Hydroxyproline assay kits Bleomycin siSTAT3 pEXPR-103-Strep vector

Adenovirus Amino acids Assay Bleomycin Catalytic domains Ccl4 Cytokines Gifts Growth factors Hydroxyproline Ires Plasmid Sirnas Smad2 Smad3 Stat3 Stat6 Strep Tgfβ1 Vector

Corresponding Organization : Seoul National University

Other organizations : Korea Institute of Science and Technology

Top 5 similar protocols

Variable analysis

independent variables

TGFβ1
Bleomycin
SiSTAT3
SiSTAT6
EPRS (1–1440 amino acids)
PRS domain of EPRS
WT STAT3
STAT6
SMAD2
SMAD3

dependent variables

Hydroxyproline (measure of fibrosis)
CCl4 (measure of fibrosis)

control variables

Cytokines and growth factors purchased from Peprotech
Hydroxyproline assay kits and CCl4 purchased from Sigma-Aldrich
Bleomycin and target-specific pooled siRNAs purchased from Santa Cruz Biotechnology
EPRS in pEXPR-103-Strep vector obtained as a gift from Dr. Myung Hee Kim
PRc/CMV-WT STAT3 previously described
PCMV-STAT6-IRES-Neo obtained as a gift from Axel Nohturfft
Adenovirus expressing SMAD2 or SMAD3 described previously

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