Whole cell extract preparation, western blotting and co-immunoprecipitation experiments were performed as described previously [5 (link),6 (link)]. Antibodies used were: anti-BrdU (ab12219, abcam), anti-FLAG (F1804, Sigma), anti-HA (HA.11 clone 16B12, Covance), anti-PCNA (ab70472, abcam).
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