Cholesterol Uptake in Macrophages

Exudate peritoneal cells were isolated from the treated nondiabetic or diabetic Apoe^−/− mice at 21 weeks of age, or db/db diabetic mice at the age of 13 weeks, 4 days after an intraperitoneal injection of thioglycolate [18] (link), [19] (link). Adherent macrophages were incubated for 18 hours with the RPMI-1640 medium containing 10 μg/ml human oxidized low-density lipoprotein (oxLDL) in the presence of 0.1 mmol/l [³H]oleate conjugated with bovine serum albumin. Cellular lipids were extracted and the radioactivity of the cholesterol [³H]oleate was determined by thin-layer chromatography [18] (link), [19] (link).