SARS-CoV-2 Spike Protein Assay

Spike protein was purchased from Sino Biologicals. Mitoxantrone was purchased from Microsource (Cat #01503278) and stored by the NCATS compound management department. Banoxantrone was purchased from Sigma (Catalog number: SML1854). pcDNA3.1-SARS-CoV-2-Spike plasmid was obtained from BEI resource^{7 (link)}. pLV-Mcherry was obtained from Addgene (Catalog number: 36084). Hoechst 33342 staining solution and proteinase K were purchased from Thermo Fisher Scientific (Catalog number: 62249 and AM2546).

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Zhang Q., Radvak P., Lee J., Xu Y., Cao-Dao V., Xu M., Zheng W., Chen C.Z., Xie H, & Ye Y. (2022). Mitoxantrone modulates a heparan sulfate-spike complex to inhibit SARS-CoV-2 infection. Scientific Reports, 12, 6294.

Publication 2022

Spike protein pLV-Mcherry Hoechst 33342 staining solution proteinase K

Banoxantrone Biologicals Hoechst 33342 Mitoxantrone Plasmid Proteinase k Sars cov 2 Sino Spike protein

Corresponding Organization : National Institutes of Health

Other organizations : Center for Biologics Evaluation and Research, United States Food and Drug Administration, National Center for Advancing Translational Sciences

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Variable analysis

independent variables

Mitoxantrone
Banoxantrone
PcDNA3.1-SARS-CoV-2-Spike plasmid
PLV-Mcherry

dependent variables

Measured outcomes (not explicitly mentioned)

control variables

Spike protein
Hoechst 33342 staining solution
Proteinase K

positive controls

PcDNA3.1-SARS-CoV-2-Spike plasmid

negative controls

Not explicitly mentioned

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