Heterologous Biosynthesis of Natural Products

Escherichiacoli DH10B (Thermo Fisher Scientific, Waltham, MA, USA) was used for cloning purposes. The basic Y. lipolytica Po1h (CLIB 882) was taken from previous work [20 (link)]. Strains were maintained as glycerol stocks at −80 °C. The plasmids pUC19 (NEB, Ipswich, MA, USA), pACYC_assembly, pKG2-PIS and pSynPfaPptAf4 [20 (link)] were used for cluster assembly. All strains and plasmids used are listed in the supplement (Additional file 1: Tables S2, S3).

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Dietrich D., Jovanovic-Gasovic S., Cao P., Kohlstedt M, & Wittmann C. (2023). Refactoring the architecture of a polyketide gene cluster enhances docosahexaenoic acid production in Yarrowia lipolytica through improved expression and genetic stability. Microbial Cell Factories, 22, 199.

Publication 2023

Escherichiacoli DH10B pUC19

Glycerol Plasmids Strains Supplement Y lipolytica

Corresponding Organization :

Other organizations : Saarland University

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Variable analysis

independent variables

Escherichia coli DH10B (Thermo Fisher Scientific, Waltham, MA, USA) for cloning purposes
Yarrowia lipolytica Po1h (CLIB 882) from previous work [20 (link)]
Plasmids pUC19 (NEB, Ipswich, MA, USA), pACYC_assembly, pKG2-PIS and pSynPfaPptAf4 [20 (link)] for cluster assembly

dependent variables

Not explicitly mentioned

control variables

Strains were maintained as glycerol stocks at −80 °C

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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