Antibody Panel for Protein Analysis

Antibodies used in the study were as follows: anti-Flag (F3165/F7425, Sigma), anti-Myc (9B11/71D10, Cell Signaling Technology (CST)), anti-HA (16B12, Biolegend and H6908, Sigma), anti-TRAF2 (sc-136999, Santa Cruz), anti-Rab5 (sc-46692, Santa Cruz), anti-Rab7 (sc-376362, Santa Cruz), anti-Sprouty 2 (sc-100862, Santa Cruz/ab85670, Abcam), anti-EGFR (sc-373746, Santa Cruz), anti-p-Tyr (9411, CST), anti-p-Ser/Thr (ab9344, Abcam and 05-368, Sigma), anti-Ser (sc-81514, Santa Cruz), anti-Thr (sc-5267, Santa Cruz), anti-Ubiquitin (sc-8017, Santa Cruz), anti-β-Actin (AC026, Abclonal), anti-GFP (AE011, Abclonal), anti-glutathione S-transferase (GST) (2622 S, CST), anti-V5 (R960-25, Thermo Fisher and 30801ES10, Yeasen), anti-Mouse/Rabbit IgG-peroxidase secondary antibody (A0545/A9044, Sigma), anti-Mouse IgG (light chain specific)-peroxidase secondary antibody (115-005-174, Jackson), and anti-DYRK1A polyclonal antibody has been previously described [30 (link)].

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Zhang P., Zhang Z., Fu Y., Zhang Y., Washburn M.P., Florens L., Wu M., Huang C., Hou Z, & Mohan M. (2021). K63-linked ubiquitination of DYRK1A by TRAF2 alleviates Sprouty 2-mediated degradation of EGFR. Cell Death & Disease, 12(6), 608.

Publication 2021

anti-Flag anti-HA H6908 anti-TRAF2 anti-Rab5 anti-Rab7 anti-Sprouty 2 ab85670 anti-EGFR ab9344 anti-Ubiquitin anti-β-Actin anti-GFP

Actin Anti antibody Anti igg Antibodies Antibody Dyrk1a Egfr Glutathione s transferase Light Mouse Peroxidase Rabbit Traf2 Ubiquitin

Corresponding Organization :

Other organizations : Tongren Hospital, Kunming University of Science and Technology, Shanghai Jiao Tong University, Stowers Institute for Medical Research, Wuhan University, Shanghai First People's Hospital

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Variable analysis

independent variables

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dependent variables

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control variables

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controls

Positive control: anti-β-Actin, anti-GFP, anti-glutathione S-transferase (GST), anti-V5
Negative control: anti-Mouse/Rabbit IgG-peroxidase secondary antibody, anti-Mouse IgG (light chain specific)-peroxidase secondary antibody

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