Validating Anti-VDR Antibody Specificity

The specificity of the anti-VDR antibody (ab3508, Abcam) which was used in immunofluorescent labeling and cell surface staining method, was checked with silencing of VDR expression via siRNA treatment in primary cortical neurons in our previous study [9 (link)]. The immune labeling of VDR protein in VDR silenced neurons was decreased almost 70% in ourprevious study [9 (link)]. This silencing efficiency was also consistent with mRNA and western blot results in our several studies [3 (link), 9 (link), 55 (link)]. Given the reliability of this anti-VDR antibody, it was chosen for cell surface staining method.

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Dursun E, & Gezen-Ak D. (2017). Vitamin D receptor is present on the neuronal plasma membrane and is co-localized with amyloid precursor protein, ADAM10 or Nicastrin. PLoS ONE, 12(11), e0188605.

Publication 2017

ab3508

Anti antibody Antibody specificity Cell Cortical Immunofluorescent Mrna Neurons Protein Sirna Staining method Western blot

Corresponding Organization : Istanbul University

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Variable analysis

independent variables

Silencing of VDR expression via siRNA treatment in primary cortical neurons

dependent variables

Immune labeling of VDR protein in VDR silenced neurons
MRNA and western blot results of VDR silencing

control variables

Not explicitly mentioned

controls

Positive control: Immune labeling of VDR protein in unsilenced primary cortical neurons
Negative control: Not mentioned

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