Protein Expression and Purification
Corresponding Organization :
Other organizations : Bristol Royal Infirmary
Variable analysis
- Transduction of CHO cells with 50 pfu/cell of RAd Fc or RAd EC4-Fc
- Protein concentration in the conditioned media
- Protein expression detected by Western blotting
- Time points for collecting conditioned media (66 and 138 hours after transduction/transfection)
- Protein purification using protein A columns
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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