CHO cells were transduced with 50 pfu/cell of RAd Fc or RAd EC4-Fc as described previously14 (link). The conditioned media was collected at 66 and 138 hours after transduction/transfection. The conditioned media was pooled and protein purification was achieved with protein A columns (Amersham Biosciences). The protein concentration was determined using the Bradford Protein assay (Sigma) and compared by Western blotting.
Free full text: Click here