Culturing MDA-MB-231, IGROV1, and UC-MSCs

MDA-MB-231 human breast cancer cells and IGROV1 human ovarian cancer cells were cultured with DMEM (high glucose) medium (Corning, Lowell, MA) supplemented with 10% fetal bovine serum (FBS) (Corning, Lowell, MA) and 1% penicillin streptomycin solution (Gibco, Rockville, MD). Medium for MDA-MB-231 cells was also supplemented with 1% MEM nonessential amino acid solution (NEAA; Gibco). UC-MSCs were isolated as described before [17 (link), 18 (link)] and cultured with DMEM/F12 medium (Gibco) containing 10% FBS (Corning), 1% penicillin streptomycin solution (Gibco), and 10 ng/ml human recombinant epidermal growth factor (EGF; Gibco). All cell lines were maintained at 37°C in a 5% CO2 incubator. To be trackable in direct coculturing model, MDA-MB-231 cells were transduced with lentiviral vector carrying green fluorescence protein (GFP) and selected with blasticidin.