Culturing Campylobacter jejuni M1cam

C. jejuni M1cam (M1 isolate [35 (link)]) was routinely cultured on Mueller–Hinton (MH) agar (Merck Life Science UK Limited, Dorset, UK) at 42 °C under microaerophilic conditions of 5% O

_{2}

, 5% CO

_{2}

, 5% H

_{2}

, 85% N₂ in an M95 variable atmosphere workstation (Don Whitley Scientific, Bingley, UK). Bacterial stocks were stored at −80 °C in MicroBank tubes (Pro-Lab Diagnostics, Birkenhead, UK). For each experiment and replicate, bacteria were always grown from freezer stocks onto fresh MH agar plates for 48 h and then restreaked and grown for a further 16 h before use.

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Stoakes E., Savva G.M., Coates R., Tejera N., Poolman M.G., Grant A.J., Wain J, & Singh D. (2022). Substrate Utilisation and Energy Metabolism in Non-Growing Campylobacter jejuni M1cam. Microorganisms, 10(7), 1355.

Publication 2022

MicroBank tubes

Agar Atmosphere Bacteria Diagnostics Replicate

Corresponding Organization : Quadram Institute

Other organizations : University of Cambridge, Oxford Brookes University

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Variable analysis

independent variables

Culture conditions (42 °C under microaerophilic conditions of 5% O2, 5% CO2, 5% H2, 85% N2)

dependent variables

Not explicitly mentioned

control variables

Mueller–Hinton (MH) agar as the culture medium
Bacterial stocks stored at -80 °C in MicroBank tubes
Bacteria grown from freezer stocks onto fresh MH agar plates for 48 h, then restreaked and grown for a further 16 h before use

controls

No positive or negative controls were explicitly mentioned

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