Human chondrocyte cell line CHON-001 derived from normal articular cartilage was obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). CHON-001 cells were grown in DMEM (Gibco, Grand Island, USA) with 10% FBS (Gibco) at 37 °C with CO2. Then, OA cellular model in vitro was established in CHON-001 cells by 24 h stimulation with 10 ng/mL IL-1β [32 (link), 33 (link)] (Sigma Aldrich, St. Louis, MO, USA).
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