Characterization of Red Blood Cell-derived Quantum Dots

High resolution transmission electron microscopy (Talos F200S G2, Thermo Scientific) was employed to analyze the morphology and size of RBCQDs with an acceleration voltage set at 200 kV. Fourier transform infrared spectrometry (Nicolet iS50, Thermo Scientific) was used to investigate the infrared absorption spectra of ginsenoside Rb1, anhydrous ethylenediamine, and RBCQDs, with samples prepared using a potassium bromide pellet method. X-ray photoelectron spectrometry (Nexsa G2, Thermo Scientific) was utilized to analyze ginsenoside Rb1 and RBCQDs with a 300 W Al K radiation source. The fluorescence spectrometer (F-4700, HITACHI) was employed to evaluate the 3D fluorescence characteristics of RBCQDs. NanoBrook 90Plus PALS (BROOKHAVEN) was used for determining the hydrodynamic diameter and potential of RBCQDs.

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Tang X., Yang X., Yu Y., Wu M., Li Y., Zhang Z., Jia G., Wang Q., Tu W., Wang Y., Zhu X, & Li S. (2024). Carbon quantum dots of ginsenoside Rb1 for application in a mouse model of intracerebral Hemorrhage. Journal of Nanobiotechnology, 22, 125.

Publication 2024

Talos F200S G2 Nicolet iS50 Nexsa G2 F-4700 NanoBrook 90Plus PALS

Corresponding Organization :

Other organizations : Nanchang University, First Affiliated Hospital of Jiangxi Medical College

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Variable analysis

independent variables

Acceleration voltage set at 200 kV (for high resolution transmission electron microscopy)

dependent variables

Morphology and size of RBCQDs (analyzed by high resolution transmission electron microscopy)
Infrared absorption spectra of ginsenoside Rb1, anhydrous ethylenediamine, and RBCQDs (investigated by Fourier transform infrared spectrometry)
X-ray photoelectron spectra of ginsenoside Rb1 and RBCQDs (analyzed by X-ray photoelectron spectrometry)
3D fluorescence characteristics of RBCQDs (evaluated by fluorescence spectrometer)
Hydrodynamic diameter and potential of RBCQDs (determined by NanoBrook 90Plus PALS)

control variables

Samples prepared using a potassium bromide pellet method (for Fourier transform infrared spectrometry)

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