Comprehensive Antibody Characterization for Transcription Research

The antibodies used were: rabbit anti-Rpb1 (sc-899; Santa Cruz Biotechnology), rabbit anti-Rpb1 (A304–405A & A304–405A, Bethyl Laboratories), rabbit anti-Rpb1 CTD pSer2 (ab5095, Abcam), rabbit anti-Spt5 (A300-868A, Bethyl Laboratories), mouse anti-Spt5 (sc-133217, Santa Cruz Biotechnology), rabbit anti-Spt5-pSer666 and -pThr806 (Twenty-first Century Century Biochemicals) previously described^{58 (link)}, rabbit anti-PP4R2 (A300–838A, Bethyl Laboratories), rabbit anti-PPP4C (A300–835A, Bethyl Laboratories), sheep anti-PP4R2-pThr173 (Division of Signal Transduction Therapy, University of Dundee Scotland)^{45 (link)}, rabbit phospho-PP1α (Thr320) antibody (2581S, Cell Signaling Technology), mouse anti-GFP (sc-9996, Santa Cruz Biotechnology), mouse anti-pan-PP1 (sc-7482, Santa Cruz Biotechnology), goat anti-PP1α (sc-6104, Santa Cruz Biotechnology), mouse anti-PP1β (sc-373782, Santa Cruz Biotechnology), rabbit anti-PP1γ (A300-906A, Bethyl Laboratories), goat anti-PP1γ (sc-6108, Santa Cruz Biotechnology), mouse anti-tubulin (T5168, Sigma-Aldrich), rabbit anti-GST (sc-459, Santa Cruz Biotechnology), mouse anti-FLAG^® M2 (F3165, Sigma-Aldrich), and rabbit anti-FLAG (2368, Cell Signaling).

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Parua P.K., Kalan S., Benjamin B., Sansó M, & Fisher R.P. (2020). Distinct Cdk9-phosphatase switches act at the beginning and end of elongation by RNA polymerase II. Nature Communications, 11, 4338.

Publication 2020

sc-899; ab5095 sc-133217, mouse anti-GFP (sc-9996, mouse anti-tubulin rabbit anti-GST mouse anti-FLAG® M2 rabbit anti-FLAG

Antibodies Antibody Goat Mouse Rabbit Sheep Therapy Tubulin

Corresponding Organization :

Other organizations : Icahn School of Medicine at Mount Sinai

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Variable analysis

independent variables

Rabbit anti-Rpb1 (sc-899; Santa Cruz Biotechnology)
Rabbit anti-Rpb1 (A304–405A & A304–405A, Bethyl Laboratories)
Rabbit anti-Rpb1 CTD pSer2 (ab5095, Abcam)
Rabbit anti-Spt5 (A300-868A, Bethyl Laboratories)
Mouse anti-Spt5 (sc-133217, Santa Cruz Biotechnology)
Rabbit anti-Spt5-pSer666 and -pThr806 (Twenty-first Century Century Biochemicals)
Rabbit anti-PP4R2 (A300–838A, Bethyl Laboratories)
Rabbit anti-PPP4C (A300–835A, Bethyl Laboratories)
Sheep anti-PP4R2-pThr173 (Division of Signal Transduction Therapy, University of Dundee Scotland)
Rabbit phospho-PP1α (Thr320) antibody (2581S, Cell Signaling Technology)
Mouse anti-GFP (sc-9996, Santa Cruz Biotechnology)
Mouse anti-pan-PP1 (sc-7482, Santa Cruz Biotechnology)
Goat anti-PP1α (sc-6104, Santa Cruz Biotechnology)
Mouse anti-PP1β (sc-373782, Santa Cruz Biotechnology)
Rabbit anti-PP1γ (A300-906A, Bethyl Laboratories)
Goat anti-PP1γ (sc-6108, Santa Cruz Biotechnology)
Mouse anti-tubulin (T5168, Sigma-Aldrich)
Rabbit anti-GST (sc-459, Santa Cruz Biotechnology)
Mouse anti-FLAG® M2 (F3165, Sigma-Aldrich)
Rabbit anti-FLAG (2368, Cell Signaling)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

The antibodies used were previously described in the reference provided (link)

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