AOM-DSS Colorectal Cancer and Colitis Models

For the CA-CRC model^{19 (link)}, 8–10 week-old C57BL/6J, A/J, or Irf1^−/− mice (The Jackson Laboratory) were injected ip with azoxymethane (AOM; 7 mg/kg) followed by three 4-day cycles of 2% (w/v) DSS in the drinking water, with each cycle 17 days apart and euthanized 6 weeks later. In some experiments, mice were injected with AOM (7 mg/kg) once, followed by two 4-days cycles of 2% (w/v) DSS and sacrificed 3 weeks later. The model of AOM-induced colorectal cancer was as previously described^{20 (link)}. For the chronic colitis model, mice were treated with three 4-days cycles of 2% (w/v) DSS and sacrificed after week 8.