HPLC analysis and fractionation were performed with the Vanquish Flex UHPLC System using the Diode Array Detector (Thermo Fisher Scientific, Waltham, MA, USA), equipped with Luna® 5 µm C18(2) 100 Å, 250 × 4.6 mm column (Phenomenex, Torrance, CA, USA).
Mass spectra were collected using maXis II 4G ETD mass spectrometer (Bruker Daltonics, Bremen, Germany) and UltiMate 3000 chromatograph (Thermo Fisher Scientific, Waltham, MA, USA), equipped with Acclaim RSLC 120 C18 2.2 µm 2.1 × 100 mm column (Thermo Fisher Scientific, Waltham, MA, USA). Spectrum registration mode: ESI ionization mode, full scan from 100–1500 m/z, MS/MS with selection of the three most intense ions, dissociation type: CID 10–40 eV, nitrogen collision gas. Mass spectra were processed using OpenChrom Lablicate Edition (1.4.0.202201211106), TOPPView v. 2.6.0 [58 (link)]. The chemical structures were identified using the GNPS [59 (link)], NPAtlas [60 (link),61 (link)] and Dictionary of Natural Products 31.1 (https://dnp.chemnetbase.com (accessed on 10 March 2022)) [38 (link)] databases.
Mass spectra were collected using maXis II 4G ETD mass spectrometer (Bruker Daltonics, Bremen, Germany) and UltiMate 3000 chromatograph (Thermo Fisher Scientific, Waltham, MA, USA), equipped with Acclaim RSLC 120 C18 2.2 µm 2.1 × 100 mm column (Thermo Fisher Scientific, Waltham, MA, USA). Spectrum registration mode: ESI ionization mode, full scan from 100–1500 m/z, MS/MS with selection of the three most intense ions, dissociation type: CID 10–40 eV, nitrogen collision gas. Mass spectra were processed using OpenChrom Lablicate Edition (1.4.0.202201211106), TOPPView v. 2.6.0 [58 (link)]. The chemical structures were identified using the GNPS [59 (link)], NPAtlas [60 (link),61 (link)] and Dictionary of Natural Products 31.1 (
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