Human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b and TENN) were originally established in Dr. Brattain’s lab in 1981 (27 (link)). All cell lines were validated by short-tandem repeat (STR) analyses at the University of Nebraska Medical Center Human DNA Identification Laboratory. STR profiles were cross-checked with the ATCC database. HCT116 and RKO cell lines with ≥ 80% match with the ATCC online STR database are considered valid (28 (link)). STR profiles of FET, CBS, HCT116b and TENN cell lines have never been reported before. Their STR profiles were confirmed in ATCC database to be of human origin and contain no mammalian interspecies contamination (Fig. S1A ). There were 5–8 passages between thawing and use in the described experiments for all the cell lines.
Cells were maintained at 37°C in a humidified incubator with 5% CO2 and cultured in McCoy’s 5A serum-free medium (M4892, Sigma, St. Louis, MO, USA) supplemented with 10 ng/ml epidermal growth factor, 20 μg/ml insulin, and 4 μg/ml transferrin (29 (link)). When the cells were subjected to growth factor deprivation stress, they were cultured in the medium in the absence of growth factor or serum supplements.
Cells were maintained at 37°C in a humidified incubator with 5% CO2 and cultured in McCoy’s 5A serum-free medium (M4892, Sigma, St. Louis, MO, USA) supplemented with 10 ng/ml epidermal growth factor, 20 μg/ml insulin, and 4 μg/ml transferrin (29 (link)). When the cells were subjected to growth factor deprivation stress, they were cultured in the medium in the absence of growth factor or serum supplements.
