pUC19 DNA was acquired from New England BioLabs
(NEB) and propagated in the Escherichia coli XL-1
Blue strain under ampicillin selection. Plasmids were purified using
a miniprep plasmid extraction kit (Qiagen). DNA CpG methyltransferase
(M.SssI) and the restriction enzymes used for DNA mapping were also
purchased from NEB. Methionine analogues were either purchased or
synthesized as described previously11 (link) and
were used as freshly prepared stock solutions adjusted to neutral
pH. Catechol O-methyltransferase (COMTase) was purchased
from Calzyme Laboratories Inc., while (−)-epinephrine (+)-bitartrate, S-(5′-adenosyl)-l -methionine (AdoMet), and S-(5′-adenosyl)-l -homocysteine (AdoHcy)
were obtained from Sigma-Aldrich. All of the other reagents were of
the highest purity available.
(NEB) and propagated in the Escherichia coli XL-1
Blue strain under ampicillin selection. Plasmids were purified using
a miniprep plasmid extraction kit (Qiagen). DNA CpG methyltransferase
(M.SssI) and the restriction enzymes used for DNA mapping were also
purchased from NEB. Methionine analogues were either purchased or
synthesized as described previously11 (link) and
were used as freshly prepared stock solutions adjusted to neutral
pH. Catechol O-methyltransferase (COMTase) was purchased
from Calzyme Laboratories Inc., while (−)-epinephrine (+)-bitartrate, S-(5′-adenosyl)-
were obtained from Sigma-Aldrich. All of the other reagents were of
the highest purity available.
