Diverse Pseudomonas aeruginosa Strains for Research

The following P. aeruginosa strains were used in this study: P. aeruginosa PAO1 (Holloway 1955 (link)), P. aeruginosa ΔpvdA ΔpchD PAO1 (ΔΔPAO1) (Visca et al. 2013 (link)) and P. aeruginosa IST27 (Leitão et al. 1996 (link)). PAO1 is a commonly used laboratory strain, whereas IST27 is a mucoid strain isolated from a cystic fibrosis patient. Other species that were used are Escherichia coli BW25113 (Grenier et al. 2014 (link)) and Salmonella enterica serovar Typhimurium NTB6 (Kortman et al. 2015 (link)). Bacteria were grown as indicated in lysogeny broth (LB; 10 g/L tryptone, 5 g/L yeast extract, 10 g/L NaCl) or Iscove’s Modified Dulbecco’s Medium (IMDM; Sigma-Aldrich). For planktonic growth experiments, pre-cultures were grown overnight in a shaking incubator at 37 °C, diluted 100-fold in fresh medium and growth was continued at 37 °C. For biofilm experiments, IMDM was supplemented with 0.5% glucose, and the biofilms were grown as indicated below.