TRPM7 Knockout Tumor Growth Evaluation

BALB/c-nu mice were purchased from Hunan SJA Laboratory Animal Co., Ltd. (Changsha, China). TRPM7 knockout T24 or WT cells (5 × 10⁶) were diluted in 200 µL of PBS and Matrigel. Tumors were evaluated and recorded regularly after cell transplantation. The volume of the tumors was measured as follows: V = D × d² × π/6, where V is volume, D is the longest diameter of the bulk tumor, and d is the shortest diameter of the bulk tumor. No mice died during the experiment.

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Wu W., Wang X., Liao L., Chen J., Wang Y., Yao M., Zhu L., Li J., Wang X., Chen A.F., Zhang G., Zhang Z, & Bai Y. (2023). The TRPM7 channel reprograms cellular glycolysis to drive tumorigenesis and angiogenesis. Cell Death & Disease, 14(3), 183.

Publication 2023

Balb c mice Bulk Cell transplantation Cells Laboratory animal Matrigel Mice Trpm7 Tumor

Corresponding Organization :

Other organizations : Third Xiangya Hospital, Central South University, Xiangya Hospital Central South University, Hunan University of Traditional Chinese Medicine, XinHua Hospital

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Variable analysis

independent variables

TRPM7 knockout T24 or WT cells

dependent variables

Tumor volume

control variables

BALB/c-nu mice
PBS and Matrigel used to dilute cells
No mice died during the experiment

controls

WT cells (as a positive control)

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