Animals were placed in an anaesthesia induction chamber, and received isoflurane
anaesthesia (5% in 6 L/min O2) until loss of reflexive responses to
hindpaw pinch (∼90 s). For induction of inflammatory pain, an intraplantar
injection of carrageenan (0.5% w/v in 0.1 mL 0.9% NaCl) was delivered to the
left hindpaw, using a 24G needle. Control rats were anaesthetised as described
above, but not injected, in order to avoid inflammation related to mechanical
injury. Behavioural testing recommenced 3 h after recovery from anaesthesia.
anaesthesia (5% in 6 L/min O2) until loss of reflexive responses to
hindpaw pinch (∼90 s). For induction of inflammatory pain, an intraplantar
injection of carrageenan (0.5% w/v in 0.1 mL 0.9% NaCl) was delivered to the
left hindpaw, using a 24G needle. Control rats were anaesthetised as described
above, but not injected, in order to avoid inflammation related to mechanical
injury. Behavioural testing recommenced 3 h after recovery from anaesthesia.
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