Human osteosarcoma (HOS, SAOS-2, 143B and U2OS) and the human osteoblast cell line hFOB1.19 were obtained from the Type Culture Collection of the Chinese Academy of Sciences. HOS, SAOS-2, 143B and U2OS cells were cultured using RPMI-1640 medium (HyClone; Cytiva) and hFOB1.19 cells were cultivated in DMEM (HyClone; Cytiva) in a humid atmosphere with 5% CO2 at 37˚C. All media were mixed with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.).
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Culturing Human Osteosarcoma Cell Lines
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Corresponding Organization :
Other organizations : The First Affiliated Hospital, Sun Yat-sen University, Sun Yat-sen University, Foshan University
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Variable analysis
independent variables
- Cell line used (HOS, SAOS-2, 143B, U2OS, hFOB1.19)
- Not explicitly mentioned
- Culture conditions (RPMI-1640 medium for HOS, SAOS-2, 143B, U2OS; DMEM for hFOB1.19)
- Incubation conditions (humid atmosphere with 5% CO2 at 37°C)
- Fetal bovine serum (10% FBS added to all media)
- No positive or negative controls explicitly mentioned
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