Cancer Cell Membrane-Coated Mesoporous Silica Nanoparticles

Cancer cell membrane–coated MSNs@ICG (CMI) was prepared according to the previous report (Fang et al., 2020 (link)). In brief, the human cervical cancer cells, Hela, were maintained in DMEM supplemented with 10% FBS, 100  U/mL penicillin G, and 100 μg/ml streptomycin. And the cells were cultured at 37°C in a humidified atmosphere of 5% CO₂. The cancer cell membrane (CCM) was extracted from human cervical cancer cells Hela by using a membrane protein extraction kit, following the instructions from the manufacturer (Biyuntian, China). The CCM was added to the MI dispersion, and the mixture was ultrasonically dispersed by a Scientz-IID ultrasonic homogenizer (Ningbo Scientz Biotechnology Co., Ltd., China) for 1 h. Afterward, CMI was extruded by a mini-extruder (Avanti, Canada) through the 100-nm polycarbonate membrane 20 times in the dark. The CCM coating of MSN@DiD (CCM/MSNs@DiD, CMD) was also prepared by the same procedure mentioned before.