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Validating miR-221 Binding Sites

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Zhou Y., Ng D.Y., Richards A.M, & Wang P. (2020). microRNA-221 Inhibits Latent TGF-β1 Activation through Targeting Thrombospondin-1 to Attenuate Kidney Failure-Induced Cardiac Fibrosis. Molecular Therapy. Nucleic Acids, 22, 803-814.
Publication 2020
psiCHECK-2 dual luciferase reporter plasmid Q5 Site-Directed Mutagenesis Kit Dual-Glo luciferase assay system
3 utr Assay Binding site Cdna Firefly luciferase Gene Luciferase Plasmid Primers Promega Renilla Site directed mutagenesis

Corresponding Organization : National University of Singapore

Other organizations : University of Otago

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