Poly-HEMA Anoikis Assay

Poly-HEMA was reconstituted in 95% ethanol to a concentration of 20 mg/mL. To prepare poly-HEMA-coated plates, poly-HEMA solution was added to cell culture plates/dishes and allowed to dry overnight in a tissue culture hood. Cells were cultured in poly-HEMA-coated plates/dishes as indicated time points, and the anoikis was analyzed by annexin V/PI staining (Pacific Blue^™ Annexin V Apoptosis Detection Kit with PI, BioLegend, San Diego, CA) according to the manufacturer’s instructions.

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li D., park Y., hemati H, & liu X. (2023). Cell aggregation prevents anoikis and induces CD44 cleavage by maintaining lipid raft integrity to promote triple negative breast cancer metastasis. Research Square.

Publication Preprint 2023

Pacific Blue™ Annexin V Apoptosis Detection Kit with PI

Annexin v Anoikis Apoptosis Cell culture Cells Dishes Ethanol Poly hema Tissue

Corresponding Organization :

Other organizations : University of Kentucky

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Variable analysis

independent variables

Poly-HEMA concentration (20 mg/mL)

dependent variables

Anoikis (analyzed by annexin V/PI staining)

control variables

Poly-HEMA-coated plates/dishes
Cells cultured in poly-HEMA-coated plates/dishes as indicated time points

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