Oxidative Stress Markers in Kidney

PARP1 activity, 8-nitroguanine, and 8-hydroxy-2′-deoxyguanosine assays were performed in whole kidneys using a universal PARP colorimetric assay kit (Trevigen, Gaithersburg, MD, USA), a 8-nitroguanine DNA/RNA damage ELISA kit (Cell Biolabs, San Diego, CA, USA) and a DNA/RNA oxidative damage ELISA kit (Cayman, Ann Arbor, MI, USA), respectively, according to the manufacturer's instructions [18 (link)19 (link)22 (link)23 (link)24 (link)25 (link)].

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, & Kim J. (2017). Spermidine is protective against kidney ischemia and reperfusion injury through inhibiting DNA nitration and PARP1 activation. Anatomy & Cell Biology, 50(3), 200-206.

Publication 2017

universal PARP colorimetric assay kit 8-nitroguanine DNA/RNA damage ELISA kit DNA/RNA oxidative damage ELISA kit

8 hydroxy 2 deoxyguanosine 8 nitroguanine A dna Assay Cayman Cell Colorimetric Dna damage Elisa Kidneys Oxidative damage Parp1

Corresponding Organization : Jeju National University

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Variable analysis

independent variables

PARP1 activity
8-nitroguanine
8-hydroxy-2′-deoxyguanosine

dependent variables

PARP1 activity
8-nitroguanine
8-hydroxy-2′-deoxyguanosine

control variables

Whole kidneys

controls

Positive controls: Not explicitly mentioned.
Negative controls: Not explicitly mentioned.

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